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J Am Acad Orthop Surg

4% paraformaldehyde-fixed brains were embedded in paraffin, and 4 m-thick sections were mounted onto MAS-GP-coated glass slides (Matsunami-glass, Osaka, Japan)

Key aspects of the dosing protocol include: Initial treatment starts with 1ml weekly for 4 weeks [9] Frequency may range from 1-3 times per week based on individual needs [1] Dosage may increase to 2ml per week or 1ml twice weekly depending on results [9] Water-soluble nature allows safe administration 1-2 times weekly [10] Some practitioners may recommend B12 shots up to twice per week specifically for energy enhancement and improved fat metabolism [11]