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Although the efficacy of tretinoin in melasma management has been established, however, a minimum treatment duration of 24 weeks is required to achieve the clinical effectiveness

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When NSBSO is reduced by GSH, the disulfide bond of NSBSO will break and NSBSO will then be converted to Nap- D F D FY-thiol and this process can be detected by liquid chromatography-mass spectrometry (LCMS) analysis (Additional file 1: Fig