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The binding affinity of GLP-1RAs to the GLP-1 receptor on rat insulinoma cells (Fuxiang Biotechnology, Shanghai, China) was measured using a human GLP1 (7-36) ELISA kit (Abcam, Waltham, MA, USA)

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Additionally, during the 68-week treatment period, although weight loss slowed, it did not plateau, suggesting that a longer treatment period may be necessary to achieve the maximum benefits of semaglutide 2.4 mg in this patient population, which this trial did not capture