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Therapy frame and future considerations Per the 2020 American College of Rheumatology guidelines, allopurinol is recommended as the initial therapeutic approach for symptomatic hyperuricemia, aiming to achieve a target serum uric acid level below 6 mg/dl

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The consistent evaluation framework includes in vitro cytotoxicity assays including MTT, CCK-8, and Trypan Blue, apoptosis and necrosis analysis, ROS quantification, haemolysis testing, and fluorescence imaging

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