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William Seeds uses the following protocols depending on which variation of CJC-1295 is being administered: CJC-1295 WITHOUT DAC: A saturation dose of 100 mcg is typically used (1 mg/kg), as any higher dosage adds minimally to the pulse of GH released CJC-1295 WITH DAC: Twice a week at 100 mcg, OR 100 mcg daily (this dose works best for short-term treatment to elevate IGF1 above physiologic levels) He also recommends avoiding food consumption 30 minutes before GHRH use and 1.5 hours after using it (due to carbs and fats blunting GH release), and therefore its best to take it either before bed (thus improving sleep) or in the morning before your first meal