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The original genetic-validation study used antisense oligonucleotide-mediated knockdown of NNMT in adipose tissue of mice on a high-fat diet, with whole-body and adipose-tissue metabolic endpoints measured against scrambled-oligonucleotide controls [2]

It also has a tyrosine residue to replace the first amino acid at the N-terminus, which researchers consider to help increase the stability of the peptide

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