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Description

Beads were washed three times with 5 ml of wash buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 20 mM imidazole, 1 g ml 1 of leupeptin, 1 g ml 1 of AEBSF HCl and 1 l benzonase, pH 8.0)

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