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1 g RNA was reverse transcribed into cDNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT, Promega) following the manufacturers instructions

Amylin is involved in the central regulation of food intake, body weight and glycaemia [5], leading to decreased food intake, reduced body weight and improved glycaemic control [1,2,3]

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199-201 Dietary patterns that support cardiovascular, metabolic, and overall health, such as a Mediterranean diet and Dietary Approaches to Stop Hypertension (DASH), have also been found to be associated with high sleep quality in most observational studies