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Anabolic effects of recombinant human growth hormone in patients with wasting associated with human immunodeficiency virus infection

Compared with GLP-1 medications, lipotropic injections are cheaper and have far fewer systemic side effects, but they also do not alter hunger hormones or gastric emptying, and they do not produce comparable results

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After incubation for 15 min at 30 C, 1,000 rpm using a 3 mm shaking diameter, cell debris was removed by centrifugation (4,400 g at 4 C for 30 min) and the supernatants were analysed by HPLCMS at two different dilutions (undiluted and four times diluted with 67% methanol, 0.2% hydrochloric acid in H 2 O)