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In experiments using AAV-DIO-hGLP1R, AAV-DIO-mGlp1r-HA and AAV-DIO-mGLP1R S33W -HA to drive receptor expression, viral titres were carefully calibrated to approximate endogenous receptor levels (Supplementary Fig

Get morning light exposure within 30 minutes of waking even on overcast days, outdoor daylight is significantly stronger than indoor lighting and helps reset your circadian rhythm Keep a consistent wake time, even at weekends this anchors your body clock more effectively than a consistent bedtime Reduce bright light and screens in the evening dim your home lighting after 8pm and consider blue-light-blocking glasses if you use screens at night 2