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There are 9 essential amino acids , including: Leucine supports muscle growth and protein synthesis Isoleucine helps regulate energy levels and muscle repair Valine important for muscle metabolism and tissue repair Lysine helps produce collagen and supports skin health Methionine supports metabolism and detoxification Phenylalanine helps produce neurotransmitters in the brain Threonine supports immune function and collagen production Tryptophan helps produce serotonin, which affects mood and sleep Histidine important for growth, tissue repair, and nerve health Common food sources of essential amino acids include eggs, dairy products, meat, fish, soy, legumes, nuts, and seeds

However, human clinical trials were primarily small, observational studies in elderly Russian patients focusing on biomarkers like cortisol, melatonin, and cholesterol