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Mitochondrial dysfunction and signaling in diabetic kidney disease: oxidative stress and beyond

The vast majority of animal studies are conducted without considering biological variables such as age, sex, and comorbidities, including hypertension, diabetes, and metabolic syndrome

B12 adequacy is directly relevant here since B12 deficiency independently causes cognitive symptoms and mood disturbance, which is another reason the B12-containing formulation is preferred for long-term use

Using an ERE-luciferase mouse, a reporter mouse that allows monitoring of ER transcriptional activity in a spatiotemporal manner, it was demonstrated that estrogen signaling is locally activated in keratinocytes in vitro (with 1 nM E2) and in vivo (with 10 nM E2)