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No human RCT has confirmed an optimal injectable schedule for cosmetic or wound-healing endpoints

The steps applied for RT-qPCR are as follows: The temperature was set to 98 C for 3 min, followed by 45 cycles of 15 s at 95 C, 30 s at the annealing temperature, 30 s at 72 C, and a melting program at a rate of 0.1 C/10 seconds between 60 and 95 C

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Simultaneously, decarboxylated S-adenosylmethionine (dcSAM), derived from Met metabolism, serves as the source of aminopropyl groups for the synthesis of spermidine and spermine (Wallace et al., 2003)