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The PC3- hTERT cell line was created by stably overexpressing the full-length hTERT cDNA in PC3 cells (Hasan et al

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Methylation levels of SFRP1 , SFRP2 , SDC2 , and PRIMA1 were determined using a MethyLight assay after cfDNA isolation and bisulfite conversion of samples ( SOX21 gene promoter methylation, serving as a fecal-based noninvasive biomarker for early diagnosis of colorectal cancer (Moradi et al., 2021)

Interestingly, semaglutide withdrawal in the STEP 1 extension was associated with SBP and DBP reversion to baseline levels