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Gene-Expression Modulation Pickart and Margolina (2018) reviewed gene-expression data indicating that GHK-Cu influences the transcription of a large set of genes involved in tissue remodeling, DNA repair, and antioxidant and inflammatory pathways, framing the apo-peptides copper-coordination chemistry as central to these reported effects [2]
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Then, primary antibody binding was detected after incubating sections with a horseradish peroxidase conjugated-secondary antibody (polyclonal anti-goat or anti-rabbit IgG HRP, R&D System, Minneapolis, MN, USA) for 2.5 h, followed by a 20 min exposure to the HRP substrate 3-amino-9-ethylcarbazole (AEC)
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