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Quantification of Cystatin C immunostaining in skin biopsies Cystatin C immunostaining in the patient biopsies was quantified by semiautomated image analysis using the ImageJ software ( Bright-field images of a section from all individuals were captured on a Nikon Eclipse 50i microscope equipped with a Nikon DS-Fil digital camera and a Nikon Digital Sight DS-U2 camera controller at a resolution of 2560 1920 pixels using a Nikon 4/0.3NA objective

doi: 10.3389/fnagi.2025.1617106 Received 23 April 2025 Accepted 30 June 2025 Published 18 September 2025 Volume 17 - 2025 Edited by Francisco Navarrete, Miguel Hernndez University of Elche, Spain Reviewed by Robert Fekete, New York Medical College, United States Yutaka Oji, Juntendo University, Japan Updates Copyright 2025 Chaudhary, Chaudhary and Rawat