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After mixing the sample or standard with ThioStar and incubating at room temperature for 15 minutes, the GSH-generated signal is read at 510 nm in a fluorescent plate reader with excitation at 390 nm

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This makes Aetna's commercial position notably different from Cigna (whose Express Scripts excludes Wegovy on the base formulary) and UnitedHealthcare (whose OptumRx covers only the CV indication on standard formulary)

In most cases, the presence of aggregates in biopharmaceutical formulations is nondesirable as it not only lowers the amount of active drug in the formulation but may also present a cytotoxicity/immunogenicity risk