DTC Experiments Cell lines derived from p48-Cre/p53F/+KrasL/+ (Sirt6 WT and KO) metastatic lung tumors (see above) were stably transduced with pLKO.1 vectors (Control and sh Gstt1 ) or iPCW-FLAG- Gstt1 (treated with doxycycline 5 days prior to injection) were harvested by trypsinization, washed twice in PBS, resuspended at 1 x 10 4 in 100ul PBS and injected into the tail vein of NOD.CB17-Prkdcscid/J mice (Jackson Laboratory) to establish lung metastases

These entities included government bodies, public and international financing organizations, and donors, as well as international implementing entities: National Agency of Water Supply, Sewerage, and Waste Infrastructure (AKUM), the Swedish Embassy SIDA, Ministry of Infrastructure and Energy, the German Development Bank (KfW), European Union Delegation, World Bank in Albania, the Swiss Embassy, Water Supply Sector Regulator Authority (ERRU), the Agency for the Management of Water Resources (AMBU), the Ministry of Health and Social Protection, the Ministry of Agriculture and Rural Development, the Ministry of Finance and Economy, the National Institute of Public Health (NIPS), The National Environment Agency (AKM), the Albanian Association of Water Supply and Sewerage (SHUKALB), Austrian Development Agency, SECO , SANE27 and SETEC-SWECO team.

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