Following incubation, caspase 3 activity was measured using the fluorogenic substrate AC-DEVD-AMC as described before ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells
F.SilvaB
They found that out of 26 microbial species that changed in CKD patients, 18 species altered with disease progression (increase in the relative abundance of Citrobacter freundii , Citrobacter werkmanii , Flavonifractor plautii , and Anaerostipes caccae and decrease in the relative abundance of Methanobrevibacter smithii , Coprococcus comes , Coprococcus eutactus , Clostridium sporogenes , Ruminococcus callidus , Ruminococcus bromii , Roseburia hominis , F
R.JafariA.Ghasemnejad-BerenjiM