57 eNOS uncoupling and altered eNOS signaling Paradoxically, in most situations in which increased oxidative stress and endothelial dysfunction are encountered, the expression of eNOS has been shown to be increased rather than decreased
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Avoid rubbing the injection site immediately afterward
For immunofluorescence (IF) staining, after deparaffinization and antigen retrieval, sections were incubated overnight at 4 C with a mouse anti-insulin antibody (1:200, GB15334, Servicebio), rabbit anti-PPAR antibody (1:50, ab23673, Abcam), or rabbit anti-UCP2 (1:200, DF8626, Affinity) antibody, followed by incubation with appropriate secondary antibodies (1:1000, 7074 S and 7076 S, CST)
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