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Hepatocyte organoids were then collected and washed using cold AdDMEM/F12 supplemented with 1% HEPES, 1% penicillin/streptomycin and 1% GlutaMAX and incubated for 10 min on ice using cold cell recovery solution (Corning, 354253) to remove the ECM

B6 mice fed with a normal fat diet (Normal Diet) or HFD (DIO) received the following treatment regimens: saline (Saline), scExe4 donor DNA-loaded LNP (Donor), or scExe4 donor DNA plus Cas9-coding DNA-loaded LNP (Donor+Cas9)