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Western blot analysis The levels of cellular proteins were quantified using Western blot analysis by utilizing primary antibodies: anti--catenin (1:1000), anti-pGSK3 (Ser9, 1:1000), anti- tGSK3 (1:1000), anti-cyclin D1 (1:1000), anti-p-Akt (1:1000), anti-tAkt (1:1000) (all from Cell Signaling Technology, Danvers, MA, USA), or anti-GAPDH (1:5000

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Post-mitotic cells, such as neurons, are particularly vulnerable to the accumulation of misfolded proteins during normal aging, concomitant with a decrease in proteasome activity

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