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enzymatic transthioesterification can be achieved by reacting glutathione with an appropriate acyl ester of coenzyme A (CoA) followed by purification from the water phase by HPLC or by chemically reacting glutathione with the corresponding acyl halide

After two freeze-thaw cycles, 20 L of 5% 5-sulfosalicylic acid (SSA) was added, followed by centrifugation at 8,000g for 10 min

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It is one of the most heavily cited peptides in preclinical tissue-repair, angiogenesis and gastrointestinal barrier research literature over the last two decades