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INS-1 832/3 were maintained in RPMI-1640 at 11 mM d-glucose, supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES, 2 mM l-glutamine, 1 mM pyruvate, 50 M -mercaptoethanol, and 1% penicillin/streptomycin 21

Human G(salpha) mutant causes pseudohypoparathyroidism type Ia/neonatal diarrhea, a potential cell-specific role of the palmitoylation cycle