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The search strategy included irritable bowel syndrome, IBS, gut microbiota, dysbiosis, and microbiome, as well as combinations of keywords related to interventions such as probiotics, prebiotics, FMT, diet, and antibiotics, and combinations of keywords related to mechanisms such as gut-brain axis, visceral hypersensitivity, intestinal permeability, immune activation, stress, and gut microbial metabolites. Our inclusion criteria prioritized: (1) high-impact studies such as systematic reviews, meta-analyses, and large, well-designed randomized controlled trials (RCTs)

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