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This physiological state is termed ER stress (12)

(2) To remove hydrophobic substances, wash the column with 3-4 bed volumes of 70% ethanol or 2 bed volumes of 1% Triton X-100 followed by 5 bed volumes of PBS (pH 7.4) washed

Over the past decade, although the mechanisms of DAMP production, their associated receptors, and downstream signaling pathways have been partially clarified, and although DAMPtargeted therapies have demonstrated potential in the treatment of inflammatory diseases and oncology, critical knowledge deficits still impede their clinical translation

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