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All mice were housed in grouped cages in a temperature-controlled environment with food and water given ad libitum

A complete blood count, antinuclear antibody (ANA), anti-double stranded DNA (dsDNA), and extractable nuclear antigen (ENA) panel was quantified along with erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), complement levels, creatinine, and glomerular filtration rate, ruling out systemic lupus erythematosus (SLE)

and STOP-Bang Questionnaire proprietary to University Health Network (Toronto, ON, Canada)

Others are in perimenopause, menopause, or dealing with hormonal changes that have made body composition much harder to control