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Cyst(e)inase is an artificial enzyme that can effectively promote GSH depletion in cells and increase intracellular lipid peroxidation levels
The non-ionic surfactant was melted using a magnetic stirrer with a heated plate (AREC, Velp Scientifica), once melted, cholesterol and DCP were added and the mixture was hydrated with a GSH solution in saline sodium chloride (5.4 M) by shaking with the magnetic stirrer for 60 min
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