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Its abnormal glycosylation modification (such as lack of N-glycosylation or insufficient O-glycosylation) can lead to hENT1 membrane localization disorder (endoplasmic reticulum degradation or intracellular retention) or decreased stability (accelerated protease degradation) (Chakraborty et al., 2018), thereby reducing gemcitabine influx and activation

All the cloned genes were fused to an N-terminal His 6 -tag following a FLAG-tag and PreScission protease cleavage site