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Quantitative PCR assays were performed using 480 SYBR Green Master reagent mix (Roche Life Science, 4707516001, murine probes) or TaqMan assays (human ETC genes) on a real-time fluorescence qPCR instrument (LightCycler 480II, Roche Life Science)

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Research also suggests that a deficiency in lipotropes can lead to liver fat accumulation and other metabolic disturbances, highlighting their potential role in supporting liver function and metabolic balance

The exception is when using very high, advanced doses of metabolic peptides, where a larger individual may need the upper end of the range