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Animals were housed and bred in a pathogen-free animal facility at the Keck School of Medicine, University of Southern California (USC), in accordance with protocols approved by the Institutional Animal Care and Use Committee

Hydration and Electrolytes Regular fluid intake and electrolyte balance are critical, especially during early weight loss

For superior sagittal sinus pressure recording, we made a single burr hole in the rostral part of the sagittal suture, above the superior sagittal sinus, and cannulated the superior sagittal sinus anterior part using a Braun intravenous cannula

GLP-1 therapy is often intended for ongoing use