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19 Immunohistochemistry For paraformaldehyde (PFA) fixation, mice were deeply anesthetized with isoflurane and perfused sequentially with heparinized normal saline, followed by 4% PFA and 0.5% glutaraldehyde

doi: 10.1016/j.cell.2015.02.020

SLC1A5 might not be an appropriate target for suppressing glutamine uptake by cancer cells because it is not the only plasma membrane glutamine transporter, and its function would therefore be compensated by other redundant glutamine transporters such as SLC38A1 and SLC38A2