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Immunoblotting Cells or tissues were lysed with RIPA lysis buffer (150 mM NaCl, 100 mM Tris, 5 mM EDTA, and 1% Triton-100) supplemented with 0.1 mmol/L PMSF (Beyotime, Shanghai, China, #ST505) and 1 mmol/L protease inhibitor cocktail (MCE, Monmouth Junction, NJ, #HY-K0010)

PMID: 38832935

A practical consideration is that patients already experiencing GLP-1-related gastrointestinal effects (bloating, constipation, loose stools) may find orlistat's side effectsincluding oily stools and urgencyparticularly bothersome when combined

Patients with AD frequently exhibit reduced gut microbial diversity (Melli et al., 2020), characterized by decreased abundance of beneficial genera such as Lactobacillus app