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Glutamine-addicted cells exhibit a decreased survival, or even undergo apoptotic cell death, associated with an increased in DNA damage, an overproduction of ROS or a decreased reduced/oxidized glutathione (GSH/GSSG) ratio

Our study has important implications for our understanding of enzymatically active and inactive glutaredoxins and could be useful for the design and optimization of artificial glutaredoxins, glutaredoxin-coupled fluorescent redox sensors and transition-state inhibitors

( a ) The expression of AHR or NRF2 in MCF-7 and U2OS cells targeted AHR and NRF2 by CRISPR/Cas9 was analyzed by Western blot