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The final pellet was re-suspended in DMEM containing penicillin, streptomycin, 10% FBS, 1 g/ml insulin (Sigma), and 20 ng/ml epidermal growth factor (EGF, Sigma)

3.5.1 CD4 T-cell iron dyshomeostasis drives SLE/LN Iron overload promotes pathogenic T-cell differentiation and accelerates disease in lupus-prone models (215): 1) In MRL/MpJ-Fas (lpr)/J (MRL/lpr) mice, iron overload expands T follicular helper (Tfh) cells, germinal center (GC) B cells, and pro-inflammatory cytokine-secreting CD4 T cells

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SCARS Center physicians recommend one at home skin check per family member per month, this includes small children and elderly family members, and an annual or biannual (twice a year) skin check with your primary care physician or dermatologist