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perfoliata were extensively washed in phosphate buffered saline to remove host material, flash-frozen, and stored at 80C until further use
Autophagy 12 , 14251428 (2016)
Its relevance here stems from a clinical reality: autoimmune thyroid disease primarily Hashimoto's affects women at seven to ten times the rate of men, and is a leading cause of hypothyroidism, fatigue, weight gain, and menstrual irregularity in women aged 3060
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