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Previous studies have demonstrated that the deficiency of monoaminergic neurotransmitters, such as 5-HT, norepinephrine (NE), and dopamine (DA), aggravates depressive behaviors and can be used as a diagnostic index for depression (Massart et al., 2012

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elegans codon-optimized BirA was synthesized with artificial introns (Genescript) and tagged at the C-terminus with GFP