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The resin was washed once with His lysis buffer and three times with His 6 wash buffer (50 mM Tris-HCl pH 7.5, 300 mM NaCl, 20 mM imidazole, 5% glycerol, 2.5 mM ME)
10.1016/s0928-4257(01)00042-0 84 StaresinicM.PetrovicI.NovinscakT.JukicI.PevecD.SuknaicS.et al (2006)
This is characterized by increased crypt depth, intact epithelial cell morphology, a higher relative density of goblet cells, and enhanced uniformity and richness of Prevotellaceae NK3B31 and Ruminococcus in the gut microbiota (Ma et al., 2023)