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Figure 3 An R2R3-MYB transcription factor has been identified as a master regulator ( The transcriptional activity of this regulator is tightly modulated by epigenetic modifications in response to sulfur availability ( Post-translational regulation occurs primarily through mitogen-activated protein kinase (MAPK)-mediated phosphorylation ( In vitro kinase assays using recombinant MPK6 and protein fragments identified a critical phosphorylation site ( 2.3 Pathway integration and compartmentalized activation Together, BCAT4, MAM1, and CYP79F1 form the core enzymatic triad of the aliphatic glucosinolate biosynthetic pathway, each executing distinct and non-redundant functions in stepwise glucoraphanin construction from amino acid precursors

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A well-characterized example of this mechanism is the Keap1Nrf2 signaling axis, illustrated in Fig