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After an additional three TBST washes, protein was visualized with SuperSignal West Dura Extended Duration Chemiluminescent Substrate (ThermoFisher) reagents following the manufacturers instructions and imaged using a ChemiDoc-It Imaging System (UVP, Upland, CA) with VisionWorks software

Oxidized PAC-quinone imine metabolites have also been shown to form GSH-conjugates which inhibit glutathione reductase (GR): the decreased activity of GR hampers the detoxification and antioxidant capacity of the GSH-GSSG cycle, further aggravating the pro-oxidative status in the cell (Ndlov et al., 2014)

Med.1987,317(10), 599-604 37