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Sterility testing was performed using direct injection technique according to European Pharmacopoeia (European Pharmacopoeia 11.0 2023 2.6.1) and United States Pharmacopeia (United States Pharmacopeia-National Formulary 2023 Issue 2, Chapter 71) guidelines, and bacterial endotoxin content measurement was performed via kinetic turbidimetry technique according to the European Pharmacopoeia (European Pharmacopoeia 11.0 2023 2.6.14) and US Pharmacopeia (US Pharmacopeia-National Formulary 2023 Issue 2, Chapter 85) guidelines

pH value and viscosity adjustment pH value adjustment: The pH value of the cream needs to be adjusted to an appropriate range (usually 5.5-7.0) to conform to the physiological environment of the skin and reduce irritation

PMID: 28287993, DOI: 10.1109/jbhi.2017.2675340

Furthermore, these medications require long-term use to sustain their benefits, leading to dependency and substantial financial costs due to their high price