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Description

Start with a lower concentration and build up tolerance over time

At 48 h after plating, the medium was replaced with DMEM supplemented with 5% horse serum (Sigma), 20 mM D-glucose and, on day 4, cytosine arabinoside (10 M) to prevent non-neuronal proliferation 33

Growing confidence in microbial biotechnology, coupled with stricter regulatory requirements surrounding additive identity, safety, and species-specific performance validation, continues supporting widespread adoption among commercial livestock producers