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Blood was collected in EDTA tubes and centrifuged at 1,600 g for 16 min at room temperature to separate the plasma, which was aspirated and stored at 80 C

Scand J Clin Lab Invest 2010

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[22] That result is the most honest way to characterize the receptor question: E-peptide biology appears to be partly IGF-1R-dependent and partly IGF-1R-independent, rather than cleanly one or the other which is why blanket claims that MGF works through its own receptor overstate what the data support