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cereus Strains WSBC 10925 and F3876/87 Bacterial pellets, harvested from cultures (100 mL) of strains WSBC 10925 and F3876/87, respectively, were extracted with EtOH (10 mL) by shaking at room temperature for 15 h, the extract was centrifuged twice (7800 g, 20 C, 12 min), the supernatant was centrifuged (18600 g, 20 C, 5 min) and membrane filtered (0.2 m

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For vial-based microdosing, concentration matters more than vial size

Future medicinal chemistry efforts may yield next-generation compounds with enhanced properties, including improved tissue selectivity, modified pharmacokinetics, or additional mechanistic activities that complement NNMT inhibition