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The lower limit of quantification (LLOQ) for dopamine was 0.5 g/ml, however, serum and nutrients in the permeability medium did not allow its quantification

According to the authors, other reported complications of coffee enemas include: thermal burns from heated coffee enemas stricture, which is an area of narrowing within the intestines bowel perforation Considerations Before considering a coffee enema, people should familiarize themselves with the potential risks of the procedure

The BBD data were analyzed using contour and 3D plots to elucidate the independent variables' effects