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Shaking creates foam air bubbles trapped against peptide molecules which can denature the peptide at the air-liquid interface

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We also found that this toxicity could be mitigated by N-acetyl-l-cysteine, an ROS inhibitor

Several aspects highlight the connection between TAO and ER oxidative stress: Autoimmune Response Induction: In TAO, thyrotropin receptor antibodies activate thyroid cell receptors, leading to hyperthyroidism and ocular complications