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Aging athletes, especially those over the age of 60, are also at risk for deficiency with age-related atrophic gastritis, low stomach acid, and an inadequate amount of a certain protein needed to absorb B12 (intrinsic factor)

SIRT1 has gained popularity as it has been linked with the French Paradox and the calorie restriction-mediated longevity and delayed incidence of several diseases associated with aging, such as cancer, atherosclerosis, and diabetes

No lethal dose was identified across a wide dose range from 0.006 mg/kg to 20 mg/kg body weight

Another possibility is that obesity could lower serum vitamin B12 levels through decreased dietary intake or absorption, increased catabolism, and sequestration in adipose tissue, or changes in the gut microbiota profiles which could affect the metabolism of vitamin B12