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Cells were washed with PBS and imaged in RMPI 1640 medium without phenol red using the confocal settings of a Leica Stellaris 8 STED FALCON in an 86X objective and a 10% power 405 nm excitation laser and emission ranges of 415-465 nm and 470-520 nm for Laurdan, plus a 5% power 647 nm excitation laser for SNAP-Surface Alexa Fluor 647

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Obesity 29(5):829836 Horber FF, Steffen R (2021) Reversal of long-term weight regain after Roux-en-Y gastric bypass using liraglutide or surgical revision

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