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The antigenantibody complex was bound to protein A/G magnetic beads for 1 h at 25 C, washed with IP Lysis/Wash buffer and purified water, eluted with the antigen/antibody complex, and analyzed using standard immunoblotting protocols

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Endurance and recovery: Research from the Journal of Strength and Conditioning Research found that athletes who used creatine experienced quicker recovery times between bouts of exercise due to enhanced ATP production