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( E H ) MDA-MB-231 cells transfected with siCTR or 2 different combination pools of 4 individual siRNA for FADS1 (siFADS1_pool1 and pool2) and FADS2 (siFADS2_pool1 and pool2) and assessed by qRT-PCR ( E ) were treated ON with 0.25 M RSL3 ( F ) or 2 h with 1 M RSL3 ( G , H ) and assayed for cell survival ( F ), confocal analysis ( G ), and cytofluorimetric analysis ( H ) to measure lipid peroxidation

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